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Human transforming growth factors induce tyrosine phosphorylation of EGF receptors

F H Reynolds, G J Todaro, C Fryling

    Nature
    |July 16, 1981
    PubMed

    Insights

    Human tumor cells secrete transforming growth factors (TGFs) that can alter cell behavior. These TGFs were found to increase phosphotyrosine levels in the epidermal growth factor (EGF) receptor.

    Area of Science:

    • Oncology
    • Cell Biology
    • Molecular Biology

    Background:

    • Cultured human tumor cell lines and RNA tumor virus-transformed cells secrete transforming growth factors (TGFs).
    • TGFs compete with epidermal growth factor (EGF) for receptor binding and can induce cell transformation in vitro.
    • Tyrosine phosphorylation activity is linked to transforming genes of tumor viruses.

    Purpose of the Study:

    • To investigate whether phosphotyrosine levels are elevated in human tumor cells.
    • To determine the effect of TGFs produced by human tumor cells on the EGF receptor.

    Main Methods:

    • Culturing human tumor cell lines.
    • Analyzing phosphotyrosine levels in tumor cells.
    • Assessing the impact of TGFs on the EGF receptor's tyrosine phosphorylation sites.

    Main Results:

    • Human tumor cells secrete TGFs.
    • TGFs produced by these cells induce phosphorylation at specific tyrosine sites on the 160,000-molecular weight (160 K) EGF receptor.

    Conclusions:

    • TGFs from human tumor cells activate tyrosine phosphorylation of the EGF receptor.
    • This finding links TGF secretion by tumor cells to alterations in EGF receptor signaling pathways.

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