Glycoprotein and protein composition of bovine leukemia virus (BLV)

Highly purified preparation of bovine leukemia virus (BLV) was obtained by the method described in this communication. BLV was isolated from cell-free culture medium, harvested from BLV-producing cell-line FLK. Polyacrylamide gel electrophoresis of BLV proteins and glycoproteins, in comparison with avian myeloblastosis virus (AMV), confirmed high purity of BLV preparations. Molecular weights of some BLV-proteins and glycoproteins were calculated. By employing polyacrylamide gradient 5 to 30% it was found that BLV structural protein p15 contains at least two components.