Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Light-induced interfacial potentials in photoreceptor membranes.

D S Cafiso, W L Hubbell

    Biophysical Journal
    |May 1, 1980
    PubMed
    Summary

    Illuminating bovine rod outer segment disk membranes causes a rapid, positive interfacial electric potential change. This light-induced potential shift, occurring at the membrane boundary, is pH- and temperature-dependent.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Paramagnetic hydrophobic ions as probes for electrically active conformational transitions in ion channels.

    Biophysical journal·2009
    Same author

    Structure and dynamics of annexin 12 bound to a planar lipid bilayer.

    Physical review letters·2003
    Same author

    Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations.

    Biochemistry·2001
    Same author

    Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 65 in helix TM1 and residues in the sequence 306-319 at the cytoplasmic end of helix TM7 and in helix H8.

    Biochemistry·2001
    Same author

    Structure and function in rhodopsin: mapping light-dependent changes in distance between residue 316 in helix 8 and residues in the sequence 60-75, covering the cytoplasmic end of helices TM1 and TM2 and their connection loop CL1.

    Biochemistry·2001
    Same author

    Transport-defective mutations alter the conformation of the energy-coupling motif of an outer membrane transporter.

    Biochemistry·2001

    Area of Science:

    • Biophysics
    • Membrane biophysics
    • Phototransduction

    Background:

    • Rod outer segment disk membranes are crucial for visual phototransduction.
    • Interfacial electric potentials play a role in membrane function.
    • Understanding light-induced changes in these membranes is key to visual neuroscience.

    Purpose of the Study:

    • To investigate the rapid interfacial electric potential changes in bovine rod outer segment disk membranes upon illumination.
    • To characterize the location, kinetics, and dependencies of this light-induced potential.
    • To elucidate the molecular mechanism underlying the observed potential change.

    Main Methods:

    • Utilized spin-labeled hydrophobic ions to detect changes in interfacial electric potential.
    • Isolated bovine rod outer segment disk membranes were subjected to controlled illumination.
    • Measured the pH and temperature dependence of the potential change.
    • Developed a kinetic model to explain the experimental observations.

    Main Results:

    • A rapid, positive interfacial electric potential change was detected at the membrane boundary upon illumination.
    • The potential change was localized to the low dielectric boundary region near the cytoplasmic surface.
    • The process exhibited a first-order half-time of approximately 7 ms at 21°C and was pH- and temperature-dependent.
    • A model involving translocation of one positive charge per bleached rhodopsin successfully explained the data.

    Conclusions:

    • Illumination of rod outer segment disk membranes induces a rapid, localized interfacial potential change.
    • This potential change is attributed to the translocation of a positive charge into the membrane boundary upon rhodopsin bleaching.
    • The findings suggest a shared molecular origin with the R2 phase of the early receptor potential.

    Related Experiment Videos