Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brain enzymes and ischemia.

R F Villa

    European Neurology
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study investigated cerebral enzyme activity changes following brain ischemia in rats. Observed enzyme modifications indicate deranged neuronal metabolism during ischemic periods, not passive degradation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Neurobiology of Depression: an Integrated Overview from Biological Theories to Clinical Evidence.

    Molecular neurobiology·2016
    Same author

    ATP-ases of synaptic plasma membranes in striatum: enzymatic systems for synapses functionality by in vivo administration of L-acetylcarnitine in relation to Parkinson's Disease.

    Neuroscience·2013
    Same author

    Energy metabolism of rat cerebral cortex, hypothalamus and hypophysis during ageing.

    Neuroscience·2012
    Same author

    Pharmacotherapy and prevention of vascular dementia.

    CNS & neurological disorders drug targets·2011
    Same author

    The effect of ischemia and pharmacological treatment evaluated on synaptosomes and purified mitochondria from rat cerebral cortex.

    Biochemical pharmacology·2010
    Same author

    Differentiated effect of ageing on the enzymes of Krebs' cycle, electron transfer complexes and glutamate metabolism of non-synaptic and intra-synaptic mitochondria from cerebral cortex.

    Journal of neural transmission (Vienna, Austria : 1996)·2006
    Same journal

    Combined Olfactory Testing and Substantia Nigra Hyperechogenicity for Diagnostic Differentiation of Parkinson's Disease.

    European neurology·2026
    Same journal

    The Role of Stroke Severity in the Association between Hypoperfusion Intensity Ratio and Futile Reperfusion after Endovascular Treatment.

    European neurology·2026
    Same journal

    The Parkinsonism of Salvador Dalí.

    European neurology·2026
    Same journal

    Disorders of Arousal and Sleep-Related Hypermotor Epilepsy in Adults: A Challenging but Necessary and Critical Distinctive Diagnosis.

    European neurology·2026
    Same journal

    Sex-Specific Phenotypic Characteristics in Obstructive Sleep Apnea: A Comprehensive Analysis of Anthropometric, Hematological, and Metabolic Profiles Stratified by Disease Severity.

    European neurology·2026
    Same journal

    Historical and Clinical Analysis of a Case of Progressive Muscular Atrophy (1853-1871).

    European neurology·2026
    See all related articles

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cellular Metabolism

    Background:

    • Cerebral enzymatic activities are crucial for energy transduction and neurotransmission.
    • Ischemic events can significantly impact brain metabolism and neuronal function.

    Purpose of the Study:

    • To assess changes in key cerebral enzyme activities after varying durations of normothermic ischemia.
    • To investigate whether observed enzyme modifications are passive or indicative of metabolic derangement.

    Main Methods:

    • Enzyme assays (lactate dehydrogenase, citrate synthase, malate dehydrogenase, NADH-cytochrome c reductase, cytochrome oxidase, glutamate dehydrogenase, acetylcholine esterase) were performed.
    • Enzyme activities were measured in mitochondrial and synaptosomal fractions from rat brain.
    • Evaluations were conducted before and after postdecapitative normothermic ischemia (5, 10, 20, 40 min).

    Related Experiment Videos

    Main Results:

    • Maximal rates of specific cerebral enzymes involved in energy transduction and neurotransmission were altered post-ischemia.
    • Modifications in enzyme activity were observed in both mitochondrial and synaptosomal fractions.
    • The pattern of changes suggests active metabolic alterations rather than simple enzyme denaturation.

    Conclusions:

    • Enzyme activity modifications following ischemia are not passive degradation.
    • These changes reflect a deranged metabolism within ischemic neurons.
    • Further research is needed to fully elucidate the complex metabolic responses to brain ischemia.