Cross-reactive anti-vesicular stomatitis virus (VSV) cytotoxic T lymphocytes recognize the major surface glycoprotein

The major surface glycoprotein (G) of vesicular stomatitis virus (VSV) was purified and incorporated into lipid vesicles containing the purified hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai Virus. These lipid vesicles were then used to modify the render target cells susceptible to lysis by anti-VSV cytotoxic T lymphocytes. The results obtained provide direct evidence that the G protein is a target antigen for specific and cross-reactive anti-VSV cytotoxic T lymphocytes. The significance of this work is discussed.