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Biological activity of Bordetella pertussis in lipopolysaccharide-resistant mice
Abstract:
Effects of Bordetella pertussis organisms, such as adjuvanticity, induction of hypersplenia, and leukocytosis as well as modification of nonspecific resistance to infection and typical morphological response of lymphatic organs, were studied in the lipopolysaccharide-resistant C3H/HeJ mouse strain. It was shown that B. pertussis exerted all of these effects in C3H/HeJ mice, although the morphological response, hypersplenia, and modification of resistance to infection with Listeria monocytogenes in such animals were less pronounced than those in lipopolysaccharide-sensitive mouse strains. This indicated that the biological activity of B. pertussis as determined in the present studies, is due partly to structural components other than lipopolysaccharide.
Insights
Bordetella pertussis impacts mice, showing effects like immune enhancement and organ changes. However, these responses were less pronounced in lipopolysaccharide-resistant mice, suggesting other bacterial components are involved.
Area of Science:
- Immunology
- Microbiology
- Pathology
Background:
- Bordetella pertussis is known to induce various immune responses.
- Lipopolysaccharide (LPS) is a key component of Gram-negative bacteria, often driving immune reactions.
- The C3H/HeJ mouse strain exhibits resistance to LPS.
Purpose of the Study:
- To investigate the effects of Bordetella pertussis in LPS-resistant C3H/HeJ mice.
- To compare these effects with those observed in LPS-sensitive mouse strains.
- To determine the role of LPS versus other bacterial components in B. pertussis-mediated effects.
Main Methods:
- Administration of Bordetella pertussis to C3H/HeJ mice.
- Assessment of adjuvanticity, hypersplenia, leukocytosis, and changes in nonspecific resistance.
- Histopathological examination of lymphatic organs.
- Comparison with LPS-sensitive mouse strains.
Main Results:
- B. pertussis induced adjuvanticity, hypersplenia, leukocytosis, and modified resistance in C3H/HeJ mice.
- These effects were less pronounced in C3H/HeJ mice compared to LPS-sensitive strains.
- Morphological changes in lymphatic organs were also observed but were less significant.
Conclusions:
- Bordetella pertussis exerts biological effects independent of its lipopolysaccharide component.
- Structural components other than LPS contribute significantly to the activity of B. pertussis.
- LPS-resistance influences the magnitude of B. pertussis-induced immune responses.