Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium-regulatory mechanisms. Functional classification using skinned fibers.

W G Kerrick, P E Hoar, P S Cassidy

    The Journal of General Physiology
    |February 1, 1981
    PubMed
    Summary

    This study used agents like adenosine 3-thiotriphosphate (ATP gamma S) and calmodulin (CaM) to classify skinned muscle fibers. Results confirm proposed calcium regulation mechanisms across different fiber types.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Selective production of rat mutant selenoprotein W with and without bound glutathione.

    Biochemical and biophysical research communications·2003
    Same author

    Dityrosine as a product of oxidative stress and fluorescent probe.

    Amino acids·2003
    Same author

    Increased prevalence of CMV gB3 in marrow of patients with aplastic anemia.

    Blood·2001
    Same author

    Abnormal contractile function in transgenic mice expressing a familial hypertrophic cardiomyopathy-linked troponin T (I79N) mutation.

    The Journal of biological chemistry·2000
    Same author

    Troponin C regulates the rate constant for the dissociation of force-generating myosin cross-bridges in cardiac muscle.

    Journal of muscle research and cell motility·2000
    Same author

    Ca(2+) measurements in skinned cardiac fibers: effects of Mg(2+) on Ca(2+) activation of force and fiber ATPase.

    Journal of applied physiology (Bethesda, Md. : 1985)·2000

    Area of Science:

    • Muscle Physiology
    • Biochemistry

    Background:

    • Calcium (Ca2+) ions are critical regulators of muscle contraction.
    • Understanding Ca2+ regulation mechanisms varies across different muscle types.

    Purpose of the Study:

    • To classify skinned muscle fiber types using specific biochemical agents.
    • To validate proposed Ca2+ regulatory mechanisms based on agent interactions.

    Main Methods:

    • Skinned muscle fibers from gizzard, mammalian striated, and scallop adductor muscles were used.
    • The effects of adenosine 3-thiotriphosphate (ATP gamma S), trifluoperazine (TFP), troponin I, protein kinase C-subunit, and calmodulin (CaM) on Ca2+-activated tension were measured.

    Main Results:

    • Agents targeting the light chain kinase-phosphatase system (ATP gamma S, TFP, C-subunit, CaM) significantly affected gizzard smooth muscle but not striated or scallop muscle.

    Related Experiment Videos

  • Troponin I inhibited Ca2+ activation in mammalian striated and gizzard fibers, but not scallop muscle.

    • Conclusions:

    • The differential effects of agents support the classification of skinned muscle fiber types.
    • Experimental results align with proposed models of Ca2+ regulation in muscle contraction.