Poliovirus-specific polypeptides in infected HeLa cells analysed by isoelectric focusing and 2D-analysis

Analysis of poliovirus-infected cells by isoelectric focusing in urea resolves at least 25 bands. Combination of isoelectric focusing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) in a two-dimensional analysis (2D-analysis) revealed a much better resolution than each method separately. By 2D-analysis most of the bands could be correlated with the known poliovirus-specific polypeptides generated in the infected cell. With this technique it was possible to determine the apparent isoelectric point (pI) of the identified poliovirus polypeptides. They are in the range from pH 5.6 for polypeptide 7d to pH 8.8 for polypeptide X, which is the most basic found so far.