Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental changes in subthreshold pace-maker currents in chick embryonic heart cells.

J R Clay, A Shrier

    The Journal of Physiology
    |March 1, 1981
    PubMed
    Summary

    Cardiac cell re-aggregates from embryonic chick hearts show developmental changes in ion currents. A decrease in background sodium current is primarily responsible for the loss of automaticity in older heart cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The inverse problem for cardiac arrhythmias.

    Chaos (Woodbury, N.Y.)·2023
    Same author

    Temperature dependence of bistability in squid giant axons with alkaline intracellular pH.

    The Journal of membrane biology·2002
    Same author

    Action potentials occur spontaneously in squid giant axons with moderately alkaline intracellular pH.

    The Biological bulletin·2001
    Same author

    A novel, kinesin-rich preparation derived from squid giant axons.

    The Biological bulletin·2001
    Same author

    Novel mechanism for Brugada syndrome: defective surface localization of an SCN5A mutant (R1432G).

    Circulation research·2001
    Same author

    Determining K+ channel activation curves from K+ channel currents.

    European biophysics journal : EBJ·2001

    Area of Science:

    • Developmental biology
    • Cardiovascular physiology
    • Cellular electrophysiology

    Background:

    • Heart cell re-aggregates from embryonic chick ventricles exhibit distinct beating properties based on developmental age.
    • Previous studies have characterized potassium ion currents in 7-day-old embryonic chick heart preparations.

    Purpose of the Study:

    • To analyze the developmental changes in membrane currents of chick embryonic heart cell re-aggregates.
    • To investigate the electrophysiological basis for the loss of automaticity during cardiac development.

    Main Methods:

    • Utilized a two-micro-electrode voltage-clamp technique to study membrane currents.
    • Analyzed preparations from 7-, 12-, and 17-day-old chick embryonic hearts within a -120 to -40 mV potential range.

    Related Experiment Videos

    Main Results:

    • Observed a developmental decrease in the amplitude of the potassium ion membrane current (1:0.2:0.03 ratio for 7-, 12-, 17-day preparations).
    • Noted alterations in the potassium current's time constant and activation potential range with development.
    • Identified a developmental decrease in inward background sodium current, correlating with reduced automaticity.

    Conclusions:

    • Developmental changes in ion currents, particularly the reduction in background sodium current, underlie the loss of automaticity in aging embryonic chick heart cells.
    • The study elucidates the electrophysiological mechanisms contributing to the maturation of cardiac automaticity.