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Related Experiment Videos

Multiple opiate receptors: phylogenetic differences.

M C Buatti, G W Pasternak

    Brain Research
    |August 10, 1981
    PubMed
    Summary

    Species exhibit varying levels and types of opioid and enkephalin binding sites. Rats possess both high and low affinity sites, while goldfish only have low affinity sites, indicating significant interspecies differences in opioid receptor distribution.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Comparative Biology

    Background:

    • Opioid and enkephalin binding sites are crucial for understanding pain modulation and reward pathways.
    • Interspecies variations in receptor characteristics can impact drug efficacy and physiological responses.

    Purpose of the Study:

    • To investigate and compare the characteristics of opioid and enkephalin binding sites across different species.
    • To determine if observed binding sites represent relevant pharmacological targets.

    Main Methods:

    • Radioligand binding assays were employed to quantify and characterize opioid and enkephalin binding sites.
    • Competitive displacement studies were performed using known opioid agonists like morphine and D-Ala2-D-Leu5-enkephalin.

    Main Results:

    • Significant differences in the levels and affinities of opioid and enkephalin binding sites were observed between species.
    • Rats displayed both high and low affinity binding sites, whereas goldfish exhibited only low affinity sites.
    • Morphine and D-Ala2-D-Leu5-enkephalin effectively displaced binding at both high and low affinity sites, irrespective of species.

    Conclusions:

    • The distribution and affinity of opioid and enkephalin binding sites vary considerably across species.
    • The presence of both high and low affinity sites in rats suggests a complex endogenous opioid system.
    • The displaceable binding indicates that these sites are pharmacologically relevant targets for opioid compounds.

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