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Related Experiment Videos

Escherichia coli gal operon proteins made after prophage lambda induction.

C R Merril, M E Gottesman, S L Adhya

    Journal of Bacteriology
    |September 1, 1981
    PubMed
    Summary

    Discoordinacy in Escherichia coli gal operon expression is caused by translational failure of the galE gene. Disrupting RNA stem-loop structures partially restored galE expression, implicating these structures in translational control.

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    The biological functions of A-kinase anchor proteins.

    Journal of molecular biology·2001

    Area of Science:

    • Molecular Biology
    • Bacteriology
    • Genetics

    Background:

    • The Escherichia coli gal operon's expression is typically coordinate.
    • Expression under the lambda phage promoter pL results in discoordinacy, with higher galK than galE expression.

    Purpose of the Study:

    • To investigate the cause of discoordinate gal operon expression from the lambda phage promoter pL.
    • To determine the role of RNA secondary structures in translational control of galE expression.

    Main Methods:

    • Constructing E. coli strains with IS1/IS2 insertions, deletions, or promoter mutations within the gal promoter region.
    • Analyzing protein synthesis to assess galE and galK expression levels.
    • Evaluating the impact of disrupting symmetrical RNA regions on translation.

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    Main Results:

    • Disruption of extensive dyad symmetry near the gal promoter partially restored galE expression.
    • This suggests that an RNA stem-loop structure interferes with ribosome binding to the galE Shine-Dalgarno sequence.
    • Complete coordinate expression of galE and galK was not achieved, indicating additional regulatory factors.

    Conclusions:

    • RNA stem-loop structures play a role in the translational discoordinacy of the E. coli gal operon under pL control.
    • Other symmetries within the transcript may also contribute to the observed translational regulation.
    • Further research is needed to fully elucidate the mechanisms governing gal operon expression from pL.