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The sequence of IS4.

R Klaer, S Kühn, E Tillmann

    Molecular & General Genetics : MGG
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    The DNA sequence of Insertion Sequence 4 (IS4) from E. coli was determined. This element has an open reading frame for 442 amino acids, providing insights into its potential transposition functions.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Insertion Sequences (IS-elements) are DNA segments with unclear transposition functions.
    • Determining IS-element functions is challenging due to multiple copies in E. coli.
    • IS-elements flanking transposons may carry transposition specificity.

    Purpose of the Study:

    • To determine the DNA sequence of IS4, an E. coli IS-element present in a single copy.
    • To investigate the potential coding capacity of IS4 for transposition functions.

    Main Methods:

    • DNA sequencing of the IS4 element.
    • Analysis of IS4 junctions with flanking chromosomal DNA at three different locations.
    • Identification of open reading frames within the IS4 sequence.

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    Main Results:

    • The complete DNA sequence of IS4 was determined.
    • An open reading frame encoding 442 amino acids was identified within IS4.
    • The junctions of IS4 with E. coli chromosomal DNA at three distinct sites were sequenced.

    Conclusions:

    • The DNA sequence of IS4 provides a basis for understanding its structure and potential function.
    • The identified open reading frame suggests IS4 may encode proteins involved in its own transposition.
    • Further genetic and physiological studies can now be conducted on the well-characterized IS4 element.