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In vitro transformation with Rous sarcoma virus and the pp60src-associated protein kinase.
Advances in Cyclic Nucleotide Research
|January 1, 1981
Summary
Investigating Rous sarcoma virus mutants revealed that pp60src protein kinase activity correlates with transformation. This kinase activity can be activated even without new protein synthesis, driving cellular changes.
Area of Science:
- Molecular Biology
- Virology
- Cell Biology
Background:
- Rous sarcoma virus (RSV) causes cell transformation through the pp60src protein.
- Understanding the precise role of pp60src kinase activity in transformation is crucial.
Purpose of the Study:
- To investigate the correlation between pp60src-associated protein kinase activity and RSV-induced transformation parameters.
- To examine the stability and activation of pp60src in transformation-defective mutants.
Main Methods:
- Analysis of fifteen transformation-defective Rous sarcoma virus mutants.
- Assaying pp60src-associated protein kinase activity.
- Monitoring cell growth control, morphology, hexose transport, and protease synthesis.
- Investigating pp60src stability and activation under varying protein synthesis conditions.
Main Results:
- pp60src kinase activity paralleled or preceded most transformation parameters, except altered cell growth control.
- Mutant pp60src did not exhibit increased turnover at non-permissive temperatures.
- Pre-existing pp60src activated upon temperature shift to permissive conditions.
- Inhibition of protein synthesis still allowed for increased kinase activity, pp60src phosphorylation, and morphological transformation.
Conclusions:
- pp60src kinase activity is a key driver of Rous sarcoma virus-induced transformation.
- pp60src activation, not necessarily its stability or de novo synthesis, is critical for transformation.
- Cellular transformation can occur even when new protein synthesis is inhibited, highlighting the direct role of pp60src.