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Related Experiment Videos

Methods compared for determining zinc in serum by flame atomic absorption spectroscopy.

J R Kelson, R J Shamberger

    Clinical Chemistry
    |February 1, 1978
    PubMed
    Summary

    Determining serum zinc (Zn) levels using flame atomic absorption spectroscopy revealed that deproteinization with trichloroacetic acid yielded higher results than simple dilution. This difference is attributed to an unidentified, nondialyzable interfering serum component affecting the dilution method.

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    Area of Science:

    • Analytical Chemistry
    • Clinical Chemistry
    • Biochemistry

    Background:

    • Accurate determination of serum zinc is crucial for diagnosing and managing various health conditions.
    • Flame atomic absorption spectroscopy (FAAS) is a common technique for measuring trace elements like zinc.
    • Different sample preparation methods can influence the accuracy of FAAS measurements.

    Purpose of the Study:

    • To compare two distinct methods for serum zinc determination using FAAS.
    • To identify potential interferences affecting serum zinc measurements.
    • To evaluate the accuracy and reliability of each method.

    Main Methods:

    • Serum samples were analyzed using two preparation techniques: simple 10-fold dilution with water and deproteinization with trichloroacetic acid (TCA).

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  • Analytical recovery of added zinc (Zn2+) was assessed for both methods.
  • Dialysis was employed to investigate the nature of interfering substances in the dilution method.

    • Main Results:

    • Both methods demonstrated high analytical recovery of added zinc (99.3% for dilution, 99.9% for TCA).
    • The TCA deproteinization method consistently yielded significantly higher serum zinc concentrations compared to the dilution method (mean difference: 91 ± 28 µg/L for 55 sera).
    • Dialysis experiments indicated interference from a nondialyzable serum component in the dilution method, which was not resolved by using a high-solids burner, 1-butanol diluent, or a manganese internal standard.

    Conclusions:

    • The simple dilution method for serum zinc determination by FAAS is subject to interference from nondialyzable serum components.
    • TCA deproteinization provides a more accurate measurement of serum zinc by FAAS, as it mitigates the interference observed in the dilution method.
    • Further investigation is needed to identify the specific interfering substance(s) in serum that affect zinc measurements by simple dilution.