Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discrete-length repeated sequences in eukaryotic genomes.

W R Pearson, J F Morrow

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Empirical determination of effective gap penalties for sequence comparison.

    Bioinformatics (Oxford, England)·2002
    Same author

    Training for bioinformatics and computational biology.

    Bioinformatics (Oxford, England)·2001
    Same author

    Flexible sequence similarity searching with the FASTA3 program package.

    Methods in molecular biology (Clifton, N.J.)·1999
    Same author

    Evolution of protein sequences and structures.

    Journal of molecular biology·1999
    Same author

    Generalized neighbor-joining: more reliable phylogenetic tree reconstruction.

    Molecular biology and evolution·1999
    Same author

    Panning for genes--A visual strategy for identifying novel gene orthologs and paralogs.

    Genome research·1999

    Researchers identified short, discrete-length DNA repeats in the silk fibroin gene. These findings suggest transposition mechanisms may drive the multiplication of repeated DNA sequences across diverse eukaryotic genomes.

    Area of Science:

    • Molecular Biology
    • Genomics
    • Evolutionary Biology

    Background:

    • Repeated DNA sequences are common in eukaryotic genomes.
    • The mechanisms of multiplication and dispersion of these sequences are not fully understood.
    • Previous studies have identified various types of repeated DNA, including transposable elements.

    Purpose of the Study:

    • To investigate the nature of repeated DNA sequences near the silk fibroin gene.
    • To explore the potential role of transposition in the proliferation of discrete-length repeated DNA.
    • To describe a method for identifying such sequences.

    Main Methods:

    • DNA hybridization techniques were used to analyze repeated DNA sequences.
    • Comparative analysis was performed with sea urchin moderately repeated sequences.

    Related Experiment Videos

  • Characterization of a novel, short repeated DNA element was conducted.

    • Main Results:

    • Two repeated DNA sequences near the silk fibroin gene hybridize with discrete-length families, constituting 0.5% of the genome.
    • A conserved-length repeated sequence was identified in sea urchin moderately repeated DNA.
    • The studied repeat is shorter (1300 bp) than typical eukaryotic transposable elements and lacks detectable terminal repetitions.

    Conclusions:

    • Discrete-length, interspersed, and sequence-faithful moderately repeated sequences suggest multiplication as discrete units.
    • Transposition mechanisms are likely responsible for the multiplication and dispersion of a significant class of repeated sequences in eukaryotes.
    • A novel type of eukaryotic transposable element, characterized by its short length and lack of terminal repeats, has been identified.