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[Morphogenesis of human rotavirus in a cell culture]
Abstract:
Morphogenesis of human rotavirus was studied by primary green monkey kidney cell culture by electron microscopy, at the levels of the 5th and 12th passage in the cell culture. The initial stages of rotavirus morphogenesis were shown to be associated with cytoplasmic inclusions. Nucleoid or core particles formed in the periphery of the inclusions were transported by budding to endoplasmic reticulum cavities where the final stages of virion maturation occurred. The loss of the outer membrane seems to be a component part of this process. Increasing number of virus passages in the cell culture exerted a certain effect on morphogenetic processes in the infected cells.
Insights
Human rotavirus morphogenesis involves cytoplasmic inclusions and endoplasmic reticulum budding. Increasing cell culture passages alter these viral development processes.
Area of Science:
- Virology
- Cell Biology
Background:
- Human rotavirus is a significant cause of gastroenteritis in children.
- Understanding rotavirus replication is crucial for developing antiviral strategies.
Purpose of the Study:
- To elucidate the morphogenesis of human rotavirus.
- To investigate the role of cell culture passages on rotavirus development.
Main Methods:
- Primary green monkey kidney cell culture.
- Electron microscopy at 5th and 12th cell culture passages.
Main Results:
- Rotavirus morphogenesis initiated in cytoplasmic inclusions.
- Core particles budded to the endoplasmic reticulum for final maturation.
- Outer membrane loss is part of virion maturation.
- Increased cell culture passages affected morphogenetic processes.
Conclusions:
- Human rotavirus assembly involves cytoplasmic and endoplasmic reticulum stages.
- Cell culture passage number influences rotavirus morphogenesis.