Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid--protein multiple binding equilibria in membranes.

J R Brotherus, O H Griffith, M O Brotherus

    Biochemistry
    |September 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of hydrochloric acid on the potential of the resting and secreting stomach.

    Federation proceedings·2010
    Same author

    The effect of applied current on the potential between a dead stomach and HCl.

    Federation proceedings·2010
    Same author

    Relationship between gastric potential and secretion when dilute saline is placed in contact with mucosa.

    Federation proceedings·2010
    Same author

    The effect of pilocarpine, mecholyl, atropine and alcohol on the gastric potential and the secretion of hydrochloric acid.

    The American journal of physiology·2010
    Same author

    On the molecular characterization of the sodium-potassium transport adenosine triphosphatase.

    The Journal of general physiology·2009
    Same author

    Advantages and limitations of spin labeling in quantitating protein-lipid associations.

    Biophysical journal·2009
    Same journal

    Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

    Biochemistry·2026
    Same journal

    Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

    Biochemistry·2026
    Same journal

    Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

    Biochemistry·2026
    Same journal

    Discovery of Bacterial Unspecific Peroxygenases.

    Biochemistry·2026
    Same journal

    Lactate Biology: Subcellular Routing and Chemical Form Define Function.

    Biochemistry·2026
    Same journal

    Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

    Biochemistry·2026
    See all related articles

    This study quantifies lipid binding sites on membrane proteins using a new equilibrium model. Results show distinct lipid affinities for cytochrome oxidase and (Na,K)-ATPase, revealing selective lipid-protein interactions.

    Area of Science:

    • Biochemistry
    • Biophysics
    • Membrane Biology

    Background:

    • Membrane proteins interact dynamically with phospholipids at their interface.
    • Understanding these lipid-protein interactions is crucial for membrane protein function.
    • Quantifying binding sites and affinities requires experimental and theoretical frameworks.

    Purpose of the Study:

    • To develop a quantitative model for lipid exchange equilibria at membrane protein interfaces.
    • To experimentally determine the number of lipid binding sites (N) and binding constants (K) for integral membrane proteins.
    • To investigate the influence of lipid head groups on binding affinities.

    Main Methods:

    • Formulation of an exchange reaction model for lipid competition at protein hydrophobic sites.

    Related Experiment Videos

  • Reconstitution of beef heart cytochrome oxidase and (Na,K)-ATPase into defined phospholipid bilayers.
  • Electron spin resonance (ESR) spectroscopy to measure lipid binding equilibria using spin-labeled phospholipids.

    • Main Results:

    • Cytochrome oxidase showed N=40 binding sites with K≈1, indicating minimal perturbation by spin-labels.
    • (Na,K)-ATPase exhibited N=60-65 sites per dimer.
    • (Na,K)-ATPase demonstrated higher binding affinity for phosphatidylserine (PS*) than phosphatidylcholine (PC*).

    Conclusions:

    • A multiple equilibria, noninteracting site binding model accurately describes lipid exchange.
    • Experimental detection of selective lipid-protein interactions is feasible through binding constant measurements.
    • This approach provides quantitative insights into the lipid annulus surrounding membrane proteins.