Cyclic nucleotides in anatomical subdivisions of the bovine lens

Adenosine 3', 5'-cyclic monophosphate (cAMP) and guanosine 3', 5'-cyclic monophosphate (cGMP) have been measured in whole and anatomically subdivided areas of bovine lenses. The highest values of cAMP and cGMP (2.843 pmoles/mg protein and 0.0139 pmoles/mg protein respectively) were found in the epithelium. In contrast, the lowest values of cAMP and cGMP (.007 pmoles/mg protein and .001 pmoles/mg protein respectively) were detected in the central nucleus. Values for the cyclic nucleotides in the whole tissue more closely resembled those of the whole nucleus.