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A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels
The Biochemical Journal
|March 1, 1981
Abstract:
1. A low-pH lithium dodecyl sulphate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. 2. Cytochrome oxidase may be partially resolved into its component polypeptides. The order of migration of these is, however, dependent on the pH of the gel system.