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Related Experiment Videos

[Insulin binding to isolated thyroid plasma membrane].

A Brisson-Lougarre, C J Blum

    Comptes Rendus Des Seances De L'Academie Des Sciences. Serie III, Sciences De La Vie
    |October 12, 1981
    PubMed
    Summary
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    Specific insulin binding sites were found in bovine thyroid plasma membranes. These findings indicate the presence of insulin receptors within the thyroid, suggesting a direct role for insulin signaling in thyroid function.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Cell Biology

    Context:

    • The thyroid gland plays a crucial role in regulating metabolism.
    • Insulin is a primary metabolic hormone, but its direct role in thyroid function is not fully understood.
    • Investigating potential insulin interactions within the thyroid is essential for a comprehensive understanding of metabolic regulation.

    Purpose:

    • To investigate the presence and characteristics of insulin binding sites in bovine thyroid plasma membranes.
    • To determine the specificity of these binding sites for insulin and related peptides.
    • To explore the kinetic properties of insulin binding to thyroid membranes.

    Summary:

    • Specific binding sites for insulin were identified in plasma membranes isolated from bovine thyroid tissue.

    Related Experiment Videos

  • Binding of radio-labeled insulin (123I-insulin) to these membranes was inhibited by unlabeled insulin and, to a lesser extent, by proinsulin.
  • Thyroid-stimulating hormone (TSH) and growth hormone (GH) did not inhibit insulin binding, indicating high specificity.
  • The dissociation kinetics of insulin binding suggested a process of negative cooperativity.
  • These results strongly suggest the presence of specific insulin receptors on bovine thyroid plasma membranes.

    • Impact:

    • Provides evidence for direct insulin signaling pathways within the thyroid gland.
    • Suggests a potential role for insulin in modulating thyroid function beyond its systemic metabolic effects.
    • Opens avenues for further research into the molecular mechanisms of insulin action in the thyroid.
    • Contributes to a deeper understanding of the interplay between insulin and thyroid hormone regulation.