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Optimized assay for serum angiotensin-converting enzyme activity.

P L Hurst, C J Lovell-Smith

    Clinical Chemistry
    |December 1, 1981
    PubMed
    Summary

    This study presents an optimized assay for measuring serum angiotensin-converting enzyme (ACE) activity. The new colorimetric method offers reliable results, unaffected by common interferences, establishing new reference intervals for men and women.

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    Area of Science:

    • Clinical Chemistry
    • Enzyme Assays
    • Biochemical Diagnostics

    Background:

    • Serum angiotensin-converting enzyme (ACE) levels are crucial biomarkers in various physiological and pathological conditions.
    • Existing assays may be subject to interferences or lack optimal precision.
    • A need exists for a robust and reliable method for quantifying serum ACE activity.

    Purpose of the Study:

    • To develop and validate an optimized two-point kinetic assay for serum angiotensin-converting enzyme (ACE).
    • To establish the performance characteristics and reference intervals for the new assay.

    Main Methods:

    • The assay utilizes a colorimetric determination of hippurate released from hippuryl-L-histidyl-L-leucine.
    • Angiotensin-converting enzyme (ACE) activity is measured in the presence of chloride ions.
    • Assay validation included assessment of precision, linearity, and interference from common substances like lipemia, icterus, and hemoglobin.

    Main Results:

    • The optimized assay demonstrated excellent precision, with within-run and between-run coefficients of variation (CVs) ranging from 2.1% to 3.2%.
    • Linearity was confirmed up to 200 U/L, indicating a wide dynamic range.
    • The method showed minimal interference from lipemia and icterus, though high hemoglobin concentrations (>1.5 g/L) caused slight negative interference.
    • Reference intervals were established as 22-82 U/L for men and 25-69 U/L for women.

    Conclusions:

    • The developed two-point kinetic assay provides a precise and reliable method for quantifying serum angiotensin-converting enzyme (ACE) activity.
    • The assay's robustness against common interfering substances enhances its clinical utility.
    • Established reference intervals aid in the accurate interpretation of serum ACE levels in patient populations.

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