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Cyclic AMP and intracellular ionic activities in Necturus gallbladder.

A Diez de los Rios, N E DeRose, W M Armstrong

    The Journal of Membrane Biology
    |January 1, 1981
    PubMed
    Summary
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    Cyclic AMP (cAMP) inhibits sodium-chloride entry in Necturus gallbladder epithelial cells. This effect on ion transport appears independent of intracellular calcium changes.

    Area of Science:

    • Cellular Physiology
    • Epithelial Transport
    • Ion Channels

    Background:

    • The Necturus gallbladder epithelium actively transports ions.
    • Cyclic AMP (cAMP) is a key intracellular second messenger regulating various cellular processes, including ion transport.

    Purpose of the Study:

    • To investigate the effects of cAMP on apical membrane potential and intracellular ion activities in Necturus gallbladder.
    • To elucidate the role of intracellular calcium in cAMP-mediated ion transport regulation.

    Main Methods:

    • Utilized open-tip and liquid ion-exchanger microelectrodes to measure apical membrane potential (Em) and intracellular activities of sodium (alphaiNa), potassium (alphaiK), and chloride (alphaiCl).
    • Monitored transepithelial potential difference (ETr) under open-circuit conditions.

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  • Administered cAMP and the calcium ionophore A23187 to serosal and mucosal media.

    • Main Results:

    • cAMP reduced alphaiCl to electrochemical equilibrium levels, decreased alphaiNa, and increased alphaiK.
    • cAMP caused a significant increase in ETr.
    • The cAMP-induced increase in alphaiK was dependent on the presence of extracellular chloride.
    • Calcium ionophore A23187 had no effect when added to the serosal medium but induced hyperpolarization and increased the driving force for chloride efflux when added to the mucosal medium.

    Conclusions:

    • cAMP inhibits coupled transapical sodium-chloride entry in Necturus gallbladder epithelial cells.
    • The observed effects of cAMP on ion transport are likely not mediated by an increase in intracellular calcium concentration.