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Herpes simplex type 1 DNA in human brain tissue.

N W Fraser, W C Lawrence, Z Wroblewska

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

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    Herpes simplex virus type 1 (HSV-1) DNA was detected in the human brain, even without clinical symptoms. This suggests HSV-1 may persist in brain tissue asymptomatically.

    Area of Science:

    • Neurovirology
    • Molecular Biology
    • Infectious Diseases

    Background:

    • Herpes simplex virus type 1 (HSV-1) establishes latent infections in human trigeminal ganglia.
    • Reactivation of HSV-1 can lead to mucocutaneous lesions and, rarely, central nervous system infections.

    Purpose of the Study:

    • To investigate the presence of HSV-1 DNA in human brain tissue from individuals without apparent HSV-1 related clinical manifestations.
    • To determine if asymptomatic individuals harbor HSV-1 in their brain tissue.

    Main Methods:

    • DNA extraction from human brain tissue samples.
    • Endonuclease digestion of extracted DNA.
    • Separation of DNA fragments using gel electrophoresis.
    • Hybridization with labeled HSV-1 DNA probes.

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  • Autoradiographic detection of hybrid bands.

    • Main Results:

    • HSV-1 DNA sequences were identified in 6 out of 11 tested human brain DNA samples.
    • Detected DNA fragments sometimes represented the complete HSV-1 genome, while others represented partial genomes.
    • Presence of terminal fragments suggested linear, nonintegrated viral DNA forms.

    Conclusions:

    • HSV-1 DNA can be present in human brain tissue asymptomatically.
    • The findings indicate a potential for subclinical HSV-1 persistence in the brain.
    • Further research is needed to understand the implications of latent HSV-1 in the brain.