Related Experiment Videos

HPLC analysis of erythrocyte pyrimidine-5'-nucleotidase inhibition by lead

R M Rocco, J L Smart, D R Brown

Research Communications in Chemical Pathology and Pharmacology

|October 1, 1981

Abstract:

The activity of pyrimidine-5'-nucleotidase (P5N) (EC 3.1.3.5) was assayed in microsamples of rat blood using high performance liquid chromotography (HPLC). The assay is based on the measurement of enzymatically formed uridine in erythrocyte hemolysates (10-50 microliter) and produces a linear activity curve from 5 to 1000 microM/g Hb/h. Acute (22 h) administration of lead acetate ip to rats induced a dose-dependent inhibition of P5N activity in erythrocyte samples.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Human papillomavirus genotypes associated with cervical cytologic abnormalities and HIV infection in Ugandan women.

Journal of medical virology·2007

Elevated manganese levels in blood and central nervous system occur before onset of clinical signs in scrapie and bovine spongiform encephalopathy.

Journal of animal science·2007

Down-regulation of MHC class I is a property common to papillomavirus E5 proteins.

Virus research·2006

Mycoplasma iguanae sp. nov., from a green iguana (Iguana iguana) with vertebral disease.

International journal of systematic and evolutionary microbiology·2006

Mycoplasma alligatoris infection promotes CD95 (FasR) expression and apoptosis of primary cardiac fibroblasts.

Clinical and diagnostic laboratory immunology·2005

Mucosal vaccination delays or prevents prion infection via an oral route.

Neuroscience·2005

See all related articles