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Identification and characterization of Kingella kingae
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|October 1, 1981
Summary
Kingella kingae, a Gram-negative bacterium, exhibits specific characteristics including beta-hemolysis and acid production from glucose. This study details its homogeneous nature and growth patterns, aiding in its identification as an opportunistic pathogen.
Area of Science:
- Microbiology
- Bacteriology
- Opportunistic Pathogens
Background:
- Kingella kingae is an opportunistic pathogen belonging to the Neisseriaceae family.
- It is rarely isolated, necessitating detailed characterization.
Purpose of the Study:
- To characterize thirteen strains of Kingella kingae, including six from Denmark.
- To elucidate the biochemical and morphological properties of this organism.
Main Methods:
- Phenotypic characterization of bacterial strains.
- Gram staining, motility tests, and biochemical assays (oxidase, catalase, saccharolytic activity).
- Assessment of growth under aerobic and anaerobic conditions.
Main Results:
- The thirteen Kingella kingae strains formed a homogeneous group.
- Organisms were small, non-motile, fastidious Gram-negative rods.
- Key characteristics included beta-hemolysis, oxidase positivity, catalase negativity, and saccharolytic activity (acid production from glucose and maltose).
- Growth was optimal aerobically, with increased colony size under anaerobic incubation.
Conclusions:
- Kingella kingae strains exhibit consistent phenotypic traits.
- Understanding these characteristics aids in the identification and study of this opportunistic pathogen.