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Sequence relationships between plasmids carrying genes for lactose utilization.

Journal of General Microbiology

|May 1, 1981

Summary

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Southern hybridization revealed the lac operon

Area of Science:

Molecular Biology
Microbiology
Genetics

Background:

The transposon Tn951 carries lac genes, which are essential for lactose metabolism.
Understanding the origin and distribution of lac genes within plasmids is crucial for microbial genetics.

Purpose of the Study:

To determine the location and orientation of the lac operon within the Tn951 transposon.
To investigate the presence and characteristics of Tn951-like sequences in naturally occurring Lac plasmids.

Main Methods:

Southern hybridization experiments were performed using pSC101::Tn951 DNA and lambda dlac DNA.
This technique was also employed to screen Lac plasmids from diverse geographical isolates.

Main Results:

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The lac operon's position and orientation within Tn951 were successfully mapped.

None of the analyzed Lac plasmids contained the complete Tn951 sequence.

However, all plasmids harbored lac genes homologous to those in Tn951, with one plasmid showing flanking sequences similar to Tn951.

Conclusions:

The lac determinants in the studied Lac plasmids and Tn951 likely originated from a common ancestral source.
The observed distribution of these lac determinants is not solely due to the spread of the Tn951 transposon itself.