Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transposon-specified site-specific recombination.

P Kitts, L Symington, M Burke

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The apparent absence of a pathway for synthesis of acetyl coenzyme A in pea chloroplasts: Lack of (14)CO 2 incorporation into lipids by the isolated organelle.

    Planta·2014
    Same author

    Massive hemoperitoneum due to ruptured ectopic gestation: postmortem CT findings in a deeply frozen deceased person.

    Legal medicine (Tokyo, Japan)·2011
    Same author

    The coincidence counting technique for orders of magnitude background reduction in data obtained with the magnetic recoil spectrometer at OMEGA and the NIF.

    The Review of scientific instruments·2011
    Same author

    Antibiotic prophylaxis in dentistry: part I. A qualitative study of professionals' views on the NICE guideline.

    British dental journal·2011
    Same author

    Antibiotic prophylaxis in dentistry: part II. A qualitative study of patient perspectives and understanding of the NICE guideline.

    British dental journal·2011
    Same author

    England tries to make polluters pay.

    Environmental science & technology·2011
    Same journal

    In This Issue.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Dolphins use names to remember whom to avoid.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Small but mighty: The outsized role of small water bodies in the global carbon cycle.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Functional traits produce conditional outcomes in different community contexts.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Cointegrate DNA molecules are key intermediates in transposition, resolved by site-specific recombination. This process is conserved across different transposable elements like gamma delta (Tn1000) and Tn501.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Transposable elements (TEs) are mobile DNA sequences.
    • Transposition involves complex molecular mechanisms, including intermediate structures.
    • Site-specific recombination plays a crucial role in resolving transposition intermediates.

    Purpose of the Study:

    • To investigate the role of cointegrate DNA molecules in the transposition of gamma delta (Tn1000) and Tn501.
    • To determine the protein requirements for the resolution of these cointegrate intermediates.
    • To explore the regulation of transposition for Tn501.

    Main Methods:

    • Analysis of DNA cointegrate structures formed during transposition.
    • Functional complementation assays using recombination proteins.

    Related Experiment Videos

  • Investigating the impact of environmental factors (Hg2+) on transposition activity.

    • Main Results:

    • Cointegrate DNA molecules containing two copies of a transposable element are resolved via site-specific recombination.
    • The gamma delta (Tn1000) transposition system produces a product interchangeable with Tn1/3tnpR protein for recombination.
    • Tn501 also forms cointegrate intermediates, with recombination activity dependent on Hg2+ presence.

    Conclusions:

    • Cointegrates are obligatory intermediates in the interreplicon transposition of Tn1/3 and Tn501.
    • The resolution of these cointegrates is mediated by site-specific recombination.
    • Tn501 transposition is regulated by the presence of mercury ions.