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Anti-inflammatory mechanism of prozime-10, a proteolytic enzyme
Abstract:
When Prozime-10 (P-10), a protease extracted from cultured both of Aspergillus melleus, was injected intravenously into anesthetized dogs, plasma ACTH was increased with a latency of 30 min, and this was followed by remarkable elevation of plasma cortisol in many instances. A similar increase in plasma cortisol was elicited after trypsin and alpha-chymotrypsin were injected. Plasma histamine was raised promptly prior to an increase in plasma ACTH after P-10 in every case. However, in certain cases, changes in cortisol occurred simultaneously with ACTH after P-10. Such a rapid elevation of cortisol can be explained, partly, by direct stimulation of the adrenal cortex by histamine.
Insights
Prozime-10, a protease from Aspergillus melleus, elevated plasma ACTH and cortisol in dogs. Histamine release preceded ACTH increase, suggesting a role in adrenal stimulation.
Area of Science:
- Enzymology
- Endocrinology
- Pharmacology
Background:
- Proteases can influence physiological processes.
- Aspergillus melleus is a source of enzymes.
- Hormonal regulation involves complex pathways.
Purpose of the Study:
- To investigate the effects of Prozime-10 (P-10) on hormonal levels in dogs.
- To explore the relationship between P-10, histamine, ACTH, and cortisol.
- To understand the mechanism of P-10-induced hormonal changes.
Main Methods:
- Intravenous injection of P-10, trypsin, and alpha-chymotrypsin into anesthetized dogs.
- Measurement of plasma ACTH, cortisol, and histamine levels.
- Analysis of temporal relationships between hormonal changes.
Main Results:
- P-10 injection led to a delayed increase in plasma ACTH and cortisol.
- Other proteases (trypsin, alpha-chymotrypsin) also elevated plasma cortisol.
- Plasma histamine increased promptly before ACTH elevation following P-10 administration.
- In some cases, cortisol changes occurred concurrently with ACTH.
Conclusions:
- P-10 stimulates the hypothalamic-pituitary-adrenal axis, increasing ACTH and cortisol.
- Histamine release appears to play a role in mediating P-10's effect on cortisol.
- Direct adrenal cortex stimulation by histamine may contribute to rapid cortisol elevation.