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Related Experiment Videos

Delayed hypersensitivity to herpes simplex virus: murine model.

R D Schrier, L I Pizer, J W Moorhead

    Infection and Immunity
    |February 1, 1982
    PubMed
    Summary

    Cell-mediated immunity is crucial for herpes simplex virus (HSV) recovery. This study developed a murine model to show that delayed hypersensitivity (DH) plays a key role in HSV immunity and protection.

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    Area of Science:

    • Immunology
    • Virology
    • Infectious Diseases

    Background:

    • Cell-mediated immunity is vital for herpes simplex virus (HSV) infection recovery.
    • Delayed hypersensitivity (DH) is a key component of cell-mediated immunity.

    Purpose of the Study:

    • To investigate the role of delayed hypersensitivity (DH) in immunity and protection against HSV.
    • To establish a murine model for studying DH in HSV infection.

    Main Methods:

    • Developed a murine model using subcutaneous HSV-1 infection and ear-swelling assay.
    • Quantified ear swelling to assess DH response kinetics and cellular infiltrate.
    • Investigated the transferability of DH using lymph node T cells, B cells, and immune serum.

    Main Results:

    • Significant ear swelling, typical of DH, was observed 3-6 days post-infection, peaking at 24 hours post-challenge.
    • Cellular infiltrate consisted predominantly of mononuclear cells.
    • DH response was virus-specific and transferable via T cells, not B cells or serum.
    • Immediate-type hypersensitivity was not detected until 15 days post-immunization.

    Conclusions:

    • The developed murine model is effective for studying DH in HSV infection.
    • DH, mediated by T cells, is a significant factor in HSV immunity.
    • This model can be used to evaluate the protective role of DH and study T cell interactions in HSV infection.

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