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Polypeptides encoded by the mer operon.

W J Jackson, A O Summers

    Journal of Bacteriology
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    This study identifies mercury resistance (HgR) polypeptides in Escherichia coli minicells. HgCl2 induces proteins of 69,000 and 12,000 daltons, with additional smaller proteins also detected.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Biochemistry

    Background:

    • Mercury resistance (HgR) in bacteria is often mediated by specific genes.
    • Understanding the proteins involved in mercury detoxification is crucial for environmental and health applications.

    Purpose of the Study:

    • To characterize the polypeptides synthesized in response to mercuric chloride (HgCl2) exposure in Escherichia coli minicells harboring HgR plasmids.
    • To identify the specific proteins encoded by mercury resistance genes and their molecular weights.

    Main Methods:

    • Escherichia coli minicells containing recombinant or natural HgR plasmids were used.
    • Cells were induced with HgCl2 and labeled with [35S]methionine.
    • Synthesized polypeptides were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

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  • Antiserum against mercuric ion reductase was used for protein identification.

    • Main Results:

    • All examined plasmids encoded two major HgCl2-inducible polypeptides of 69,000 and 12,000 daltons.
    • Additional HgCl2-inducible proteins in the 14,000- to 17,000-dalton range were frequently observed.
    • Mercuric ion reductase antiserum reacted with the 69,000-dalton polypeptide and a minor 66,000-dalton protein.
    • Analysis of recombinant plasmids revealed five HgCl2-inducible polypeptides, including the 69,000, 66,000, 15,100, 14,000, and 12,000 dalton proteins.

    Conclusions:

    • Escherichia coli HgR plasmids encode multiple polypeptides that are induced by HgCl2.
    • The 69,000-dalton polypeptide is likely mercuric ion reductase, with smaller proteins potentially involved in mercury resistance mechanisms.
    • Specific DNA fragments of the mer operon encode distinct sets of mercury-inducible proteins.