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Genetic and nucleotide sequence homologies in bacillus genomes
Molecular & General Genetics : MGG
|January 1, 1981
Summary
Investigating Bacillus subtilis strains revealed variations in the aromatic amino acid cluster organization. These differences in the tryptophan (trp) operon structure correlate with strain history and gene activity.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- The organization of metabolic pathways, such as the aromatic amino acid cluster, can vary between bacterial strains.
- Understanding these variations is crucial for bacterial genetics and strain characterization.
Purpose of the Study:
- To examine the divergence in the aromatic amino acid cluster organization among heterogenetic Bacillus subtilis strains.
- To investigate the structural variations within the tryptophan (trp) operon across different B. subtilis genomes.
Main Methods:
- Utilized hybridization techniques with a trp homolog from Bacillus pumilus.
- Employed marker survival analysis following restriction enzyme digestion (ECoRI and HindIII).
- Compared genomic cleavage site locations and gene transforming activity.
Main Results:
- Identified variations in the location of ECoRI and HindIII cleavage sites within the trp operon of B. subtilis strains W23, 3610, and 168M.
- Observed a correlation between the relative transforming activity of surviving genes and the historical lineage of the strains.
- Demonstrated divergence in the aromatic amino acid cluster organization.
Conclusions:
- The trp operon organization exhibits significant divergence among heterogenetic Bacillus subtilis strains.
- Genomic variations, including restriction enzyme cleavage sites, reflect the evolutionary history and functional differences of these strains.