Use of plaque assays to study thyroglobulin autoantibody synthesis by human peripheral blood lymphocytes

Plaque assays have been used to study thyroglobulin autoantibody synthesis and total immunoglobulin production by cultures of peripheral blood lymphocytes from patients with Hashimoto's thyroiditis. Freshly isolated Hashimoto or normal lymphocytes contained small numbers (7-1300) of total IgG plaque-forming cells (PFC) and total IgM PFC, but specific thyroglobulin antibody PFC was undetectable. After 5-8 days' culture with pokeweek mitogen (PWM), total IgG and IgM PFC were markedly increased to a geometric mean of 10,140 (8414-12,220) IgG PFC and 3450 (163-7534) IgM PFC per 10(6) cultured lymphocytes (95% confidence limits in parentheses). Furthermore, a mean of 63 (22-287) specific IgG thyroglobulin antibody PFC per 10(6) lymphocytes were detectable in cultures of Hashimoto lymphocytes. The IgG thyroglobulin antibody PFC were stimulated by Epstein-Barr virus (EBV) infection, suggesting that EBV infection may be useful in obtaining monoclonal thyroid autoantibodies.