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Lung phosphatidate phosphatase: activity during altered physiologic states.

D A Filler, R A Rhoades

    Experimental Lung Research
    |February 1, 1982
    PubMed
    Summary

    Lung phosphatidic acid phosphatase (PAPase) activity significantly increases after birth in rats. This enzyme

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    Area of Science:

    • Biochemistry
    • Pulmonology
    • Developmental Biology

    Background:

    • Phosphatidic acid phosphatase (PAPase) is crucial for lipid metabolism.
    • Its role in lung surfactant production during development is not fully understood.
    • Lung microsomal PAPase activity was investigated across different developmental stages.

    Purpose of the Study:

    • To characterize rat lung microsomal PAPase activity.
    • To determine the enzyme's response to physiological changes during development and under altered maternal conditions.

    Main Methods:

    • Microsomal fractions from fetal, neonatal, and adult rat lungs were isolated.
    • PAPase activity was measured using phosphatidic acid as a substrate.
    • Enzyme activity was assessed at optimal pH (7.0) and substrate concentration (1.0 mM).

    Main Results:

    • PAPase activity was higher in fetal rat lungs compared to liver.
    • A significant 53% increase in PAPase activity was observed immediately following birth.
    • Activity remained unchanged in fetuses exposed to maternal diabetes, fasting, or premature breathing.

    Conclusions:

    • Rat lung microsomal PAPase activity dramatically increases post-birth.
    • The enzyme's activity is not sensitive to maternal diabetes, fasting, or premature breathing.
    • PAPase may not be a key regulatory enzyme in lung surfactant phospholipid synthesis.

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