Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Naphthalene plasmids in pseudomonads.

M A Connors, E A Barnsley

    Journal of Bacteriology
    |March 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a rapid method to detect naphthalene plasmids in pseudomonads. Specific plasmids, NAH2 and NAH3, were found to determine naphthalene metabolism and transferability, influencing catechol pathways.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fractalkine: a novel angiogenic chemokine in rheumatoid arthritis.

    The American journal of pathology·2001
    Same author

    Evidence of IL-18 as a novel angiogenic mediator.

    Journal of immunology (Baltimore, Md. : 1950)·2001
    Same author

    Regulation of angiogenesis by the C-X-C chemokines interleukin-8 and epithelial neutrophil activating peptide 78 in the rheumatoid joint.

    Arthritis and rheumatism·2001
    Same author

    IL-4 adenoviral gene therapy reduces inflammation, proinflammatory cytokines, vascularization, and bony destruction in rat adjuvant-induced arthritis.

    Journal of immunology (Baltimore, Md. : 1950)·2001
    Same author

    Specificity constants for two-substrate reactions.

    The Biochemical journal·1993
    Same author

    Phosphoinositide metabolism in the developing conceptus. Effects of hyperglycemia and scyllo-inositol in rat embryo culture.

    Diabetes·1992
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Pseudomonads are known for their metabolic versatility.
    • Naphthalene metabolism in bacteria is often plasmid-mediated.
    • Understanding plasmid roles is crucial for microbial genetics.

    Purpose of the Study:

    • To develop a rapid method for detecting naphthalene plasmids in pseudomonads.
    • To characterize the plasmids responsible for naphthalene metabolism.
    • To investigate the transferability and regulation of associated enzymes.

    Main Methods:

    • Direct lysis of bacteria in alkaline sodium dodecyl sulfate.
    • Plasmid DNA extraction and analysis.
    • Conjugation experiments for plasmid transfer.

    Related Experiment Videos

  • Restriction fragment analysis (BamHI, HindIII) and DNA hybridization.

    • Main Results:

    • A rapid lysis method successfully detected plasmids in naphthalene-metabolizing pseudomonads.
    • Strains NCIB 9816 and PG contained plasmids (NAH2 and NAH3) essential for naphthalene metabolism.
    • These plasmids were transferable via conjugation and influenced catechol pathway enzymes.
    • NAH2 and NAH3 showed distinct restriction patterns but hybridized with NAH, suggesting relatedness.

    Conclusions:

    • Naphthalene metabolism in these pseudomonads is primarily determined by specific, transferable plasmids.
    • The identified plasmids (NAH2, NAH3) regulate both naphthalene degradation and catechol pathway enzymes.
    • The rapid lysis method is effective for detecting naphthalene plasmids in bacterial strains.