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[Morphological study of a mixed alphavirus infection]
Voprosy Virusologii
|November 1, 1981
Summary
Venezuelan equine encephalomyelitis virus and other arboviruses can form multiploid virions during mixed cultivation. These mixed infections also induce changes in progeny virions and polymorphic shapes.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Context:
- Investigating viral interactions and genetic exchange during co-infection.
- Utilizing paired cultivation techniques to study virus progeny.
- Focusing on arboviruses such as Venezuelan equine encephalomyelitis virus, Semliki Forest virus, and Sindbis virus.
Purpose:
- To determine the frequency of multiploid virion formation in mixed viral infections.
- To observe alterations in virion morphology and shape in virus progeny.
- To explore the potential for genetic reassortment and phenotypic changes in co-cultivated viruses.
Summary:
- Combined cultivation of Venezuelan equine encephalomyelitis virus resulted in multiploid virions in 17.8% of cases.
- Subsequent mixed infections with Semliki Forest and Sindbis viruses showed progeny virion changes in 20% and 16.6% of cases, respectively.
- Mixed cultivation of Sindbis and Semliki Forest viruses yielded multiploid virions in 42.8% of cases, with some exhibiting polymorphic shapes (rounded, oval, elongated, triangular).
Impact:
- Demonstrates that mixed viral infections can lead to the formation of abnormal virion structures.
- Highlights the potential for increased viral genetic diversity and adaptation through co-cultivation.
- Provides insights into the mechanisms of viral evolution and the generation of novel viral variants.