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Studies on Proteins of simian sarcoma-associated virus with different growth history
Abstract:
Simian sarcoma-associated virus (SSaV) was repeatedly passaged on three human cell lines. The proteins of the progeny virus were analysed for the presence of variant polypeptides. Occasionally, a few variant polypeptides were observed. One-dimensional peptide maps of the major virus protein p30 revealed no modifications after 25 cycles of infection on the three cell lines studied. The peptide map of Pr65gag of virus grown through 25 passages on a human chondrosarcoma cell line was slightly different from that of the virus stock before passaging. The relative amount of the virus protein p30 as compared to p18 and p16 (possibly the SSaV equivalents of p15E and p12E) was variable depending on the host cell. Virus grown on Daudi cells was relatively deficient in p18 and p16. These virus particles were morphologically altered and had a low infectivity.
Insights
Simian sarcoma-associated virus (SSaV) adaptation in human cells yielded minor protein changes. Viral protein Pr65gag showed slight peptide map differences after extensive passaging, impacting infectivity.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Simian sarcoma-associated virus (SSaV) is a retrovirus.
- Understanding viral adaptation to host cells is crucial for virology research.
Purpose of the Study:
- To investigate protein alterations in Simian sarcoma-associated virus (SSaV) after repeated passaging in human cell lines.
- To analyze the impact of host cell adaptation on viral protein structure and infectivity.
Main Methods:
- Repeatedly passaging SSaV on three distinct human cell lines.
- Analyzing progeny virus proteins using techniques like one-dimensional peptide mapping.
- Comparing viral protein profiles before and after extensive passaging.
Main Results:
- Most viral proteins showed no significant modifications after 25 infection cycles.
- The major virus protein p30 remained unchanged.
- The Pr65gag protein exhibited slight peptide map differences after passaging on a chondrosarcoma cell line.
- Relative amounts of viral proteins varied by host cell, with Daudi cells showing deficiency in p18 and p16.
- Virus grown on Daudi cells displayed morphological alterations and reduced infectivity.
Conclusions:
- SSaV adaptation in human cells can lead to subtle protein modifications, particularly in Pr65gag.
- Host cell-dependent variations in viral protein composition affect viral morphology and infectivity.
- These findings contribute to understanding retroviral adaptation and host-pathogen interactions.