Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA conformation, dynamics, and interactions in solution.

D J Patel, A Pardi, K Itakura

    Science (New York, N.Y.)
    |May 7, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pf1 filamentous phage as an alignment tool for generating local and global structural information in nucleic acids.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Antibacterial properties of penicilli cultures.

    Sicilia medica·2010
    Same author

    Hemodynamics.

    Annual review of physiology·2009
    Same author

    Structural biology of RNA silencing and its functional implications.

    Cold Spring Harbor symposia on quantitative biology·2007
    Same author

    Self-rated quality of life in celiac disease.

    Digestive diseases and sciences·2004
    Same author

    1H-1H correlations across N-H...N hydrogen bonds in nucleic acids.

    Journal of biomolecular NMR·2002
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Nuclear magnetic resonance (NMR) reveals how DNA sequence affects structure and flexibility. This study details DNA-drug interactions and their impact on DNA conformation and dynamics.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Structural Biology

    Background:

    • DNA conformation and dynamics are crucial for biological processes.
    • Understanding DNA-drug interactions is key to developing therapeutics.

    Purpose of the Study:

    • To investigate the structure and dynamics of a DNA duplex and its modified analogs.
    • To examine the binding of actinomycin and Netropsin to DNA, both individually and simultaneously.
    • To elucidate the role of nucleic acid sequence in DNA structure and flexibility.

    Main Methods:

    • Nuclear Magnetic Resonance (NMR) spectroscopy in aqueous solution.
    • Analysis of chemical shift and Nuclear Overhauser Effect (NOE) parameters for structural information.
    • Line width and saturation recovery experiments to probe kinetics at the base pair level.

    Related Experiment Videos

    Main Results:

    • Detailed structural and dynamic information was obtained for the DNA duplex and its analogs.
    • Binding sites and conformational changes induced by actinomycin and Netropsin were identified.
    • The influence of DNA sequence on flexibility and conformational transitions was clarified.
    • The transmission of perturbations to adjacent base pair regions was estimated.

    Conclusions:

    • NMR spectroscopy is a powerful tool for studying DNA conformation, dynamics, and drug interactions.
    • Nucleic acid sequence significantly influences DNA structure, flexibility, and conformational dynamics.
    • DNA-binding drugs induce specific structural and dynamic alterations that can propagate through the helix.