Murine leukemia virus-mediated transformation of a mouse epithelial cell line MMC-E

Mouse embryo epithelial cells, MMC-E, were malignantly transformed in culture with MuLV derived from C3H/MCA 5 cells chemically induced by 5-iododeoxyuridine. The frequency of transformation was significantly enhanced if infection was followed by treatment with 100 ng of 12-O-tetradecanoylphorbol-13-acetate per ml. The virus-transformed cells retained their high plating efficiency in soft agar in the absence of 12-O-tetradecanoylphorbol-13-acetate and formed neoplasms in nude mice.