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Herpes simplex vaccine using cell membrane associated antigen in an animal model
Developments in Biological Standardization
|January 1, 1981
Summary
Researchers created non-infectious Herpes simplex virus type 2 (HSV-2) vesicles from infected cells. These vesicles were antigenic and successfully protected guinea pigs against HSV-2 infection.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Herpes simplex virus type 2 (HSV-2) is a significant human pathogen.
- Developing effective vaccines against HSV-2 remains a challenge.
Purpose of the Study:
- To investigate the potential of cell-derived membrane vesicles as a vaccine candidate against HSV-2.
- To assess the immunogenicity and protective efficacy of these vesicles.
Main Methods:
- Herpes simplex virus type 2 (HSV-2) infected Vero cells were treated with dithiothreitol and formaldehyde.
- Plasma membrane vesicles containing no detectable live virus were isolated.
- These preparations were injected into guinea pigs.
- Animals were subsequently challenged intravaginally with HSV-2.
Main Results:
- The prepared vesicles were found to be antigenic.
- Injection of these vesicles induced an immune response in guinea pigs.
- The vaccinated guinea pigs showed protection against intravaginal HSV-2 challenge.
Conclusions:
- Cell-derived membrane vesicles, devoid of live virus, can be effectively prepared.
- These non-infectious vesicles possess significant antigenicity.
- Such preparations demonstrate protective potential against HSV-2 infection, suggesting their utility as a vaccine strategy.