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Mouse hepatitis virus immunofluorescence in formalin- or Bouin's-fixed tissues using trypsin digestion
Laboratory Animal Science
|February 1, 1982
Summary
This study demonstrates a reliable immunofluorescence method for detecting mouse hepatitis virus antigens in fixed tissues. The technique is effective even with aged tissue samples, aiding in definitive diagnosis.
Area of Science:
- Veterinary Pathology
- Immunohistochemistry
- Virology
Background:
- Mouse hepatitis virus (MHV) is a significant pathogen in laboratory mice.
- Accurate diagnosis of MHV infection is crucial for research integrity.
- Traditional diagnostic methods may have limitations in sensitivity or sample preservation.
Purpose of the Study:
- To establish and validate an immunofluorescence technique for detecting MHV antigens.
- To evaluate the impact of different fixation methods and storage conditions on antigen detection.
- To provide a practical diagnostic tool for MHV infections.
Main Methods:
- Immunofluorescence staining was performed on formalin- and Bouin's-fixed tissues.
- Tissues underwent routine histopathological processing and partial trypsin digestion.
- Staining efficacy was assessed in relation to fixation type and paraffin block storage duration.
Main Results:
- Immunofluorescence successfully detected MHV viral antigens in fixed tissues.
- Formalin fixation yielded superior staining compared to Bouin's fixation.
- Staining intensity remained consistent in paraffin-embedded sections stored for up to 2 years at room temperature.
Conclusions:
- Trypsin-enhanced immunofluorescence is a valuable method for MHV diagnosis.
- The technique is robust, utilizing readily available reagents and standard histopathology procedures.
- This method offers a practical and reliable approach for definitive MHV diagnosis in research settings.