Related Experiment Video

Updated: Sep 2, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Isolation of human oncogene sequences (v-fes homolog) from a cosmid library

J Groffen, N Heisterkamp, F Grosveld

    Science (New York, N.Y.)
    |June 4, 1982
    PubMed

    Insights

    Researchers identified human cellular sequences homologous to the feline sarcoma virus (FeSV) v-fes gene. These human sequences, when transfected into cells, did not show transforming activity, suggesting they are not oncogenic.

    Area of Science:

    • Molecular Biology
    • Oncogenomics
    • Virology

    Background:

    • Feline sarcoma virus (FeSV) contains transforming sequences (v-fes gene) common to Gardner (GA) and Snyder Theilen (ST) isolates.
    • Understanding the human homolog of viral oncogenes is crucial for cancer research.

    Purpose of the Study:

    • To identify and characterize the human homolog(s) of the v-fes gene found in FeSV.
    • To determine if the human v-fes homolog possesses transforming activity.

    Main Methods:

    • Construction of a human lung carcinoma DNA library in a cosmid vector system.
    • Isolation and characterization of cosmid clones containing GA/ST FeSV v-fes homologous sequences.
    • Analysis of sequence homology and colinearity, including identification of potential introns.
    • Transfection of isolated human c-fes sequences into RAT-2 cells using a thymidine kinase selection system.

    Main Results:

    • Three cosmid clones were isolated, containing approximately 56 kilobases of contiguous human DNA with GA/ST FeSV v-fes homologous sequences.
    • The homologous sequences were distributed discontinuously over a 9.5-kilobase region, indicating the presence of at least three introns.
    • Transfection of the human c-fes sequence into RAT-2 cells demonstrated no detectable transforming activity.

    Conclusions:

    • The human genome contains sequences homologous to the FeSV v-fes gene.
    • The identified human c-fes sequence lacks the transforming potential observed in the viral v-fes gene.
    • This suggests that the human homolog may function differently or require additional factors for oncogenic transformation.

    More Related Videos

    HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

    HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

    Published on: March 31, 2019

    Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing09:49

    Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

    Published on: July 5, 2019

    Related Concept Videos

    DNA Isolation01:24

    DNA Isolation

    DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...