[The presence of 3,4-benzopyrene in plants I. Methods]

Purification methods of vegetable extracts have been experienced during our researches on the possible presence of the aromatic polycyclic hydrocarbons (particularly 3,4 benzopyrene) in vegetables. The validity of different methods has been determined by IR spectrophotometric analysis using special apparatus (IR-DATA-STATION). Vegetables extracts have been purified efficaciously by column chromatography, saponification and special methods (Grimmer's method). The described method eliminates, nearly entirely, the polluting substances that could alternate the chemical analysis of the vegetable extracts.