Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA organization in nucleosomes.

D P Bazett-Jones, F P Ottensmeyer

    Canadian Journal of Biochemistry
    |March 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Electron spectroscopic imaging visualizes DNA in nucleosomes, confirming two supercoil turns per core. Active gene chromatin shows altered DNA organization, lacking this structure until salt concentration changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structure and function of the perinucleolar compartment in cancer cells.

    Cold Spring Harbor symposia on quantitative biology·2011
    Same author

    Changes in chromatin fiber density as a marker for pluripotency.

    Cold Spring Harbor symposia on quantitative biology·2010
    Same author

    Identification of a molecular recognition feature in the E1A oncoprotein that binds the SUMO conjugase UBC9 and likely interferes with polySUMOylation.

    Oncogene·2010
    Same author

    Freestanding lipid bilayers as substrates for electron cryomicroscopy of integral membrane proteins.

    Journal of microscopy·2002
    Same author

    UV-induced binding of ING1 to PCNA regulates the induction of apoptosis.

    Journal of cell science·2001
    Same author

    Ca(2+) shuttling in vesicles during tip growth in Neurospora crassa.

    Fungal genetics and biology : FG & B·2001
    Same journal

    Purification and characterization of dihydrofolate reductase from Walker 256 carcinosarcoma.

    Canadian journal of biochemistry·1982
    Same journal

    Structure of the glycopeptides of a human gamma 1-immunoglobulin G (Tem) myeloma protein as determined by 360-megahertz nuclear magnetic resonance spectroscopy.

    Canadian journal of biochemistry·1982
    Same journal

    Phosphorylated and unphosphorylated forms of cardiac tropomyosin.

    Canadian journal of biochemistry·1982
    Same journal

    Glycerol-induced adenine nucleotide catabolism in rat liver cells.

    Canadian journal of biochemistry·1982
    Same journal

    Large-scale production of citrate synthase from a cloned gene.

    Canadian journal of biochemistry·1982
    Same journal

    Analysis of the expression of cloned genes using an Escherichia coli cell-free system.

    Canadian journal of biochemistry·1982
    See all related articles

    Area of Science:

    • Molecular Biology
    • Chromatin Structure
    • Electron Microscopy

    Background:

    • Nucleosomes are the basic units of DNA packaging in chromatin.
    • Understanding DNA organization within nucleosomes is crucial for gene regulation.
    • Previous models proposed approximately two supercoil turns of DNA per nucleosome core.

    Purpose of the Study:

    • To directly visualize DNA organization within nucleosomes using electron spectroscopic imaging.
    • To examine the structure of nucleosomes from transcriptionally active genes.
    • To investigate the effect of salt concentration on chromatin structure.

    Main Methods:

    • Electron spectroscopic imaging (ESI) for direct DNA visualization.
    • Dark field electron microscopy.

    Related Experiment Videos

  • Fractionation of chromatin using DNAase II sensitivity and MgCl2 solubility.

    • Main Results:

    • ESI confirmed approximately two supercoil turns of DNA wound around the nucleosome core in bulk chromatin.
    • Nucleosomes from active genes exhibited a less distinct beaded appearance and altered phosphorus distribution.
    • This altered structure lacked the recognizable two-turn supercoil organization per subunit.
    • The structure of active gene chromatin was reversibly changed by altering NaCl concentration.

    Conclusions:

    • Direct visualization of DNA within nucleosomes is achievable with electron spectroscopic imaging.
    • Transcriptionally active chromatin displays a distinct structural organization compared to bulk chromatin.
    • The DNA organization within active chromatin is salt-dependent and differs from the canonical two-turn supercoil model.