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Related Experiment Videos

Tn292l, a transposon encoding fosfomycin resistance.

J M García-Lobo, J M Ortiz

    Journal of Bacteriology
    |July 1, 1982
    PubMed
    Summary

    Researchers identified a new transposable element, Tn2921, responsible for fosfomycin resistance in Serratia marcescens. This element was successfully transferred to other plasmids, demonstrating its function in conferring antibiotic resistance.

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    Construction and evaluation of an ORFeome-based Brucella whole-genome DNA microarray.

    Microbial pathogenesis·2009

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Antibiotic resistance is a growing global health concern.
    • Fosfomycin is an important antibiotic used to treat various bacterial infections.
    • Understanding the genetic basis of fosfomycin resistance is crucial for developing effective treatment strategies.

    Purpose of the Study:

    • To identify and characterize the genetic element responsible for fosfomycin resistance in Serratia marcescens plasmid pOU900.
    • To investigate the transferability and function of this resistance determinant in different plasmid contexts.

    Main Methods:

    • Transposition of the fosfomycin resistance determinant from pOU900 into ColE1 and RP4 plasmids.
    • Transformation of host bacteria lacking RecA function.
    • Analysis of DNA fragments, size, and restriction patterns to confirm the presence of the transposable element.

    Main Results:

    • A novel 7.5-megadalton transposable element, named Tn2921, was identified.
    • Tn2921 conferred fosfomycin resistance upon its successful transposition into ColE1 and RP4 plasmids.
    • The acquisition of resistance correlated with the presence of a distinct, uniformly sized DNA fragment with a consistent restriction pattern.

    Conclusions:

    • Tn2921 is a distinct transposable element encoding fosfomycin resistance.
    • This element can be mobilized and confer resistance in different plasmid systems.
    • A preliminary map of Tn2921 was generated, paving the way for further structural and functional studies.

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