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Interference among defective interfering particles of vesicular stomatitis virus
Journal of Virology
|January 1, 1982
Summary
Defective interfering (DI) particles of vesicular stomatitis virus (VSV) exhibit hierarchical replication advantages over standard VSV and each other. These differences are linked to unique 3' end RNA sequences influencing polymerase and N-protein binding.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Defective interfering (DI) particles are viral mutants that require a standard virus for replication.
- Vesicular stomatitis virus (VSV) is a model system for studying RNA virus replication and interference.
Purpose of the Study:
- To investigate the replication hierarchy and interference mechanisms of different vesicular stomatitis virus (VSV) defective interfering (DI) particles.
- To determine the role of terminal RNA sequences in DI particle replication and interference.
Main Methods:
- Infection of cells with combinations of parental VSV and three distinct DI particles (DI 0.52, DI 0.45, DI-T).
- Analysis of RNA genome replication and accumulation of plus and minus RNA templates.
- Sequencing of 3' terminal regions of DI RNAs.
Main Results:
- A hierarchical replication sequence was established: DI 0.52 > DI 0.45 ≤ DI-T > standard VSV.
- Interference correlated with altered ratios of intracellular RNA templates and novel 3' end sequences in DI genomes.
- DI 0.52 particles possessed greater 3' end complementarity compared to DI-T and DI 0.45 particles.
Conclusions:
- Replication advantage and interference are not solely due to multiplicity or site occupancy.
- Terminal RNA sequences, specifically at the 3' end, are critical for VSV DI particle interference.
- Two potential mechanisms, polymerase and N-protein binding affinities, are proposed to explain interference patterns based on terminal RNA sequences.