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Multimeric forms of herpes simplex virus type 2 glycoproteins.
Journal of Virology
|January 1, 1982
Summary
Herpes simplex virus type 2 glycoproteins, including gA, gB, and gC, were found to exist in multimeric forms. This discovery provides new insights into the structural organization of these viral proteins.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Herpes simplex virus type 2 (HSV-2) is a significant human pathogen.
- Viral glycoproteins play crucial roles in HSV-2 infection and pathogenesis.
- Understanding the structural forms of HSV-2 glycoproteins is essential for developing antiviral strategies.
Purpose of the Study:
- To investigate the potential for multimeric forms of HSV-2 specific glycoproteins.
- To characterize the molecular weight and composition of these potential multimers.
Main Methods:
- Analysis of detergent extracts from HSV-2 infected cells.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions and at lower temperatures.
- Identification of high-molecular-weight glycosylated species.
Main Results:
- Two high-molecular-weight glycosylated species were detected.
- A 275,000 molecular weight species identified as a multimer of gA or gB glycoproteins (or both).
- A 230,000 molecular weight species identified as a multimeric form of the gC glycoprotein.
Conclusions:
- HSV-2 gC, gA, and gB glycoproteins can exist in a multimeric form.
- These findings contribute to understanding the structural complexity of HSV-2.
- Further research into these multimeric forms may reveal new therapeutic targets.