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Related Experiment Videos

Mapping class I gene sequences in the major histocompatibility complex.

L R Pease, S G Nathenson, L A Leinwand

    Nature
    |July 22, 1982
    PubMed
    Summary
    This summary is machine-generated.

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    The major histocompatibility complex (MHC) class I genes in mice are more extensive than previously thought, with new sequences mapping to the Qa-T1a region and centromeric to the K locus.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Genomics

    Background:

    • The major histocompatibility complex (MHC) class I glycoproteins are crucial for immune responses.
    • These proteins are encoded by a multigene family located on mouse chromosome 17.
    • Previous studies indicated a complex structure, but the full extent was unknown.

    Purpose of the Study:

    • To investigate the comprehensive structure of the mouse MHC class I multigene family.
    • To map the genetic locations of additional class I gene sequences.
    • To explore the evolutionary divergence of different domains within class I glycoproteins.

    Main Methods:

    • DNA was isolated from various standard, congeneic, and MHC recombinant mouse strains.
    • Restriction enzyme digestion and Southern blot hybridization with H-2 cDNA probes were employed.

    Related Experiment Videos

  • Analysis of restriction patterns using amino-terminal and carboxy-terminal probes.

    • Main Results:

    • Identified and mapped numerous polymorphic MHC class I gene sequences.
    • Several class I gene sequences were localized to the Qa-T1a region, outside the traditional H-2 complex.
    • One gene sequence was mapped centromeric to the K locus.
    • Different restriction patterns were observed for amino-terminal and carboxy-terminal probes, suggesting domain-specific evolution.

    Conclusions:

    • The mouse MHC class I multigene family is more extensive than previously recognized.
    • New class I gene sequences reside in regions beyond the classical H-2 complex.
    • Exons encoding different domains of class I glycoproteins may have undergone distinct evolutionary pathways.