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Related Experiment Videos

Plasmids from Staphylococcus aureus replicate in yeast Saccharomyces cerevisiae.

R Goursot, A Goze, B Niaudet

    Nature
    |July 29, 1982
    PubMed
    Summary

    Staphylococcus aureus plasmids can replicate in yeast, demonstrating a broad host range across prokaryotes and eukaryotes. This finding suggests potential similarities in replication mechanisms between these diverse organisms.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Some plasmids, like RP4, replicate in diverse Gram-negative bacteria.
    • Small Staphylococcus aureus plasmids exhibit a broad host range, replicating in Gram-positive and Gram-negative bacteria (e.g., Escherichia coli).

    Purpose of the Study:

    • To investigate the replication capability of Staphylococcus aureus plasmids in a lower eukaryote, Saccharomyces cerevisiae (yeast).
    • To explore the phylogenetic breadth of host organisms for Staphylococcus aureus plasmids.

    Main Methods:

    • Construction of hybrid plasmids combining a Staphylococcus aureus plasmid (pC194) with an Escherichia coli plasmid (YIp5) containing a selectable yeast marker (ura-3).
    • Transformation of yeast Saccharomyces cerevisiae with these hybrid plasmids.

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  • Assessment of plasmid maintenance and stability in yeast.

    • Main Results:

    • Hybrid plasmids demonstrated high transformation efficiency in yeast.
    • Plasmids were maintained extrachromosomally in yeast.
    • No significant modification of the plasmids was observed during their residence in yeast.

    Conclusions:

    • Staphylococcus aureus plasmids are capable of replication in yeast (Saccharomyces cerevisiae).
    • This suggests that prokaryotic and eukaryotic replication signals may share similarities.
    • The study expands the known host range of Staphylococcus aureus plasmids up the phylogenetic tree into eukaryotes.