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Membrane currents in the rabbit atrioventricular node cell.

S Kokubun, M Nishimura, A Noma

    Pflugers Archiv : European Journal of Physiology
    |March 1, 1982
    PubMed
    Summary
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    Researchers studied rabbit atrioventricular (A-V) node cells, identifying slow inward currents (is) and potassium currents essential for action potentials. This research clarifies ionic mechanisms underlying slow conduction in the A-V node.

    Area of Science:

    • Cardiovascular Physiology
    • Cardiac Electrophysiology
    • Ion Channel Function

    Background:

    • The atrioventricular (A-V) node plays a crucial role in cardiac impulse conduction.
    • Understanding the ionic basis of A-V node action potentials is vital for explaining cardiac arrhythmias and conduction delays.

    Purpose of the Study:

    • To investigate the ionic currents responsible for action potential generation and propagation in the rabbit A-V node.
    • To characterize the properties and ionic selectivity of identified currents, including slow inward current (is) and hyperpolarization-activated current (ih).

    Main Methods:

    • Dissection of rabbit A-V node into small specimens (0.2 x 0.2 x 0.2 mm).
    • Voltage clamp experiments using the two-microelectrode technique.
    • Application of pharmacological agents (D600, TTX) and ionic manipulations (Na+-free, Ca2+-free Tyrode solution, varied K+ concentration).

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    Main Results:

    • Spontaneous action potentials were recorded in small A-V node specimens.
    • A transient inward current (is), blocked by D600 and sensitive to Na+ and Ca2+, was identified.
    • A delayed outward current, carried by K+ ions, and a hyperpolarization-activated inward current (ih) were observed.
    • Tetrodotoxin (TTX) inhibited the upstroke of anodal break excitation, indicating a contribution of sodium current (iNa).

    Conclusions:

    • The rabbit A-V node exhibits distinct ionic current systems, including slow inward current (is), potassium outward current, and hyperpolarization-activated current (ih).
    • These currents, along with a TTX-sensitive sodium current (iNa), contribute to the unique electrophysiological properties and slow conduction within the A-V node.
    • The findings provide insights into the ionic mechanisms governing cardiac impulse propagation in the A-V node.