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Inverted repeats of Tn5 are transposable elements.

D E Berg, L Johnsrud, L McDivitt

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1982
    PubMed
    Summary

    The kanamycin-resistance transposon Tn5 contains two transposable elements, IS50-L and IS50-R. DNA analysis reveals IS50

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • The kanamycin-resistance transposon Tn5 is a key element in genetic studies.
    • Understanding the transposition mechanism of Tn5 is crucial for its application.

    Purpose of the Study:

    • To investigate the transposable nature of the inverted repeats within Tn5.
    • To characterize the DNA sequence and transposition properties of IS50.

    Main Methods:

    • DNA sequence analysis of IS50.
    • Experimental determination of transposition efficiency of IS50 ends.

    Main Results:

    • Each 1.5-kilobase inverted repeat of Tn5, designated IS50-L and IS50-R, is independently transposable.
    • IS50 is 1533 base pairs long and generates 9-bp direct repeats.
    • The ends of IS50 feature an 8-of-9-bp inverted repeat, with outer ends exhibiting higher activity than inner ends.

    Conclusions:

    • IS50-L and IS50-R are functional, independently transposable units within Tn5.
    • Transposition activity is asymmetric, with outer ends being more efficient.
    • A transposase recognition site likely extends beyond the conserved 8 bp at the outer ends of IS50.

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